Cell trapping method, method for producing specific cell- trapping device, and method for producing specific cell-containing solution

A method for producing a specific cell-trapping device for producing a specific cell-trapping device which selectively traps a specific cell included in a cell-containing solution at a filter surface by filtering a liquid using a cell trapping device, the cell trapping device including a housing which has an introduction region having a predetermined volume and for introducing a liquid selected from the cell-containing solution containing two or more types of cells and a treating solution for treating the cells in the cell-containing solution into an interior, and a discharge flow path for discharging the liquid to the exterior;

• and a filter which is provided on a flow path between the introduction region and the discharge flow path and in which a plurality of through holes for the liquid to flow through is formed in the thickness direction, the method comprising:
• a step of draining a liquid to the cell trapping device for n (n is a natural number) times,
• wherein from the first step of draining the liquid to the n-th step of draining the liquid, a liquid surface of the liquid in the introduction region on the filter is maintained at a predetermined liquid surface height, and when the n-th step of draining the liquid is completed, the discharging of the liquid from the cell trapping device is stopped in a state where the liquid surface is at a predetermined height in the filter.

A method for producing a specific cell-containing solution obtained by selectively trapping a specific cell included in a cell-containing solution at a filter surface by filtering a liquid using a cell trapping device, the cell trapping device including a housing which has an introduction region having a predetermined volume and for introducing a liquid selected from the cell-containing solution containing two or more types of cells and a treating solution for treating the cells in the cell-containing solution into an interior, and a discharge flow path for discharging the liquid to the exterior;

• and a filter which is provided on a flow path between the introduction region and the discharge flow path and in which a plurality of through holes for the liquid to flow through is formed in the thickness direction, the method comprising:
• a step of draining a liquid to the cell trapping device for n (n is a natural number) times,
• wherein from the first step of draining the liquid to the n-th step of draining the liquid, a liquid surface of the liquid in the introduction region on the filter is maintained at a predetermined liquid surface height, and when the n-th step of draining the liquid is completed, the discharging of the liquid from the cell trapping device is stopped in a state where the liquid surface is at a predetermined height in the filter, and
• wherein after the step of draining a liquid to the cell trapping device, a step of collecting a liquid existing in the housing is included.