Back to results
US 2017/0281828 A1Published application
Three-dimensional bioprinted artificial cornea
AssigneeThe Regents of the University of California
InventorsKang ZHANG, Shaochen CHEN, Xin QU, Hong OUYANG
Life of this patent
Estimating the term…
Abstract
An artificial cornea is fabricated by separately culturing live stromal cells, live corneal endothelial cells (CECs) and live corneal epithelial cells (CEpCs), and 3D bioprinting separate stromal, CEC and CEpC layers to encapsulate the cells into separate hydrogel nanomeshes. The CEC layer is attached to a first side of the stromal layer and the CEpC layer to a second side of the stromal layer to define the artificial cornea.
Claims26
A method for fabricating an artificial cornea, comprising:
- culturing live stromal cells;
- 3D bioprinting a stromal layer encapsulating the live stromal cells into a first hydrogel nanomesh;
- culturing live corneal endothelial cells (CECs);
- 3D bioprinting a CEC layer encapsulating the live CECs into a second hydrogel nanomesh;
- culturing live corneal epithelial cells (CEpCs);
- 3D bioprinting a CEpC layer encapsulating the live CEpCs into a third hydrogel nanomesh;
- and
- attaching the CEC layer to a first side of the stromal layer and the CEpC layer to a second side of the stromal layer.
5 more elements
An artificial cornea, comprising:
- a layered structure comprising:
- a 3D bioprinted stromal layer comprising live stromal cells encapsulated into a first hydrogel nanomesh, the stromal layer having a first side and a second side;
- a 3D bioprinted CEC layer comprising live corneal endothelial cells (CECs) encapsulated into a second hydrogel nanomesh;
- a 3D bioprinted CEpC layer comprising live corneal epithelial cells (CEpCs) encapsulated into a third hydrogel nanomesh;
- and
- wherein the CEC layer is attached to the first side of the stromal layer and the CEpC layer is attached to the second side of the stromal layer.
3 more elements
24-26. (canceled)
28-30. (canceled)
Dependencies are inferred from the claim text.