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US 2017/0281686 A1Published application
Bone marrow stromal cell derived extracellular matrix protein extract and uses thereof
AssigneeStembiosys, Inc.
InventorsRogelio Zamilpa, Edward S. Griffey
Life of this patent
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Abstract
Disclosed are bone marrow stromal cell derived extracellular matrix protein extracts that are useful for the expansion and proliferation of mesenchymal stem cells and for various therapeutic applications.
Claims18
An extracellular matrix (ECM) protein extract comprising:
- a bone marrow stromal cell derived ECM grown on a substrate and comprising insoluble and soluble proteins, wherein the ECM is not attached to the substrate on which it was grown, and wherein all or a portion of the soluble proteins originally present in the ECM have been removed.
An extracellular matrix (ECM) protein extract made by the method comprising:
- (a) obtaining viable bone marrow stromal cells;
- (b) culturing the bone marrow stromal cells on a substrate to produce a 3D ECM on the substrate;
- (c) decellularizing the bone marrow stromal cells from the ECM;
- (d) physically removing the ECM from the substrate;
- (e) contacting the ECM with an aqueous component with agitation to dissolve and disassociate the soluble proteins of the ECM;
- and
- (f) removing the aqueous component from the remaining insoluble portion (protein extract) of the ECM.
4 more elements
A method of making an extracellular matrix (ECM) protein extract, the method comprising:
- (a) obtaining viable bone marrow stromal cells;
- (b) culturing the bone marrow stromal cells on a substrate to produce a 3D ECM on the substrate;
- (c) decellularizing the bone marrow stromal cells from the ECM;
- (d) physically removing the ECM from the substrate;
- (e) contacting the ECM with an aqueous component with agitation to dissolve and disassociate the soluble proteins of the ECM;
- and
- (f) removing the aqueous component from the remaining insoluble portion (protein extract) of the ECM.
4 more elements
Dependencies are inferred from the claim text.