Cryopreservation instrument and method of using same
AssigneeResearch Institute At Nationwide Children's Hospital
InventorKenneth John McLaughlin
Life of this patent
Estimating the term…
Abstract
A cryopreservation instrument and method of using is provided. The instrument includes a substantially planar elongated member comprising a cryosafe material. The elongated member has a first end and a second end. The first end comprises a cap insert dimensioned and configured to be fit in the interior recess of a vial cap, the second end comprises a biological contact portion configured to accommodate biological material.
Claims24
A cryopreservation instrument, comprising:
- a substantially planar elongated member comprising a cryosafe material, the elongated member having a first end and a second end,
- the first end comprising a cap insert dimensioned and configured to be fit in the interior recess of a vial cap, the second end comprising a biological contact portion configured to accommodate biological material.
The cryopreservation instrument of claim l, wherein the cap insert comprises at least a portion having a width greater than an internal diameter of the vial cap.
A cryopreservation instrument, comprising:
- a substantially planar elongated member comprising a cryosafe material, the substantially planar elongated member comprising a first end being dimensioned and configured to be removably receivable within a recess portion of a cap with little relative clearance;
- and
- a second end configured to receive a biological specimen, the first end and second end defining an axis.
A method of preserving a sample, comprising:
- providing a cryopreservation instrument comprising a substantially planar elongated member having a first end and a second end, the first end comprising a cap insert dimensioned and configured to be fit in the interior recess of a vial cap, the second end comprising a biological contact portion configured to accommodate biological material;
- inserting the cap insert, into an externally threaded vial cap comprising an interior recess, to produce a cryo-tool having a cap end and the biological contact portion;
- loading biological material onto the biological contact portion of the cryo-tool;
- placing the biological contact portion of the cryo-tool into liquid nitrogen to obtain cryopreserved biological material;
- maintaining the cryopreserved biological material on the cryo-tool and placing the cryo-tool into an internally threaded vial being threadably engagable with the vial cap;
- allowing cryopreserved biological material to equilibrate with the conditions in the vial;
- and
- screwing the vial cap onto the vial to obtain a sealed vial.
The method of claim, 12, wherein the cap insert comprises a resilient, deformable or elastic material.
A cryopreservation instrument, comprising:
- a substantially planar elongated member comprising a cryosafe material, the elongated member having a first end and a second end,
- the first end comprising a cap insert dimensioned and configured to be fit in the interior recess of a vial cap, the second end comprising a biological contact portion configured to accommodate biological material;
- and
- the first end further comprising substantially parallel first and second guides symmetrically spaced from said biological contact portion.
Dependencies are inferred from the claim text.