Dominant mutation in the tdm gene leading to diplogametes production in plants
AssigneeInstitut National de la Recherche Agronomique
InventorsRaphaël Mercier, Marta Cifuentes, Laurence Cromer
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Abstract
The invention relates to a dominant mutation in the TDM gene leading to the production of 2n gametes in plants, to the plants comprising said mutation, and to their use in plant breeding. The invention relates also to plants in which the dominant mutation in the TDM gene is combined with the inactivation of a gene involved in meiotic recombination in plants and a gene involved in the monopolar orientation of the kinetochores during meiosis. These plants which produce apomeiotic gametes are also useful in plant breeding.
Claims16
A method for obtaining a plant producing Second Division Restitution 2n gametes,
- wherein said method comprises providing, by random or targeted mutagenesis or by genetic transformation, a plant comprising a dominant mutation within a gene, herein designated as TDM gene, coding for a protein designated herein as TDM protein,
- wherein said protein has at least 75% sequence identity with amino acid residues 1 to 286 of the TDM protein of SEQ ID NO:
- 1 when said plant is Brassica spp. or at least 30% identity with said residues when said plant is different from Brassica spp, and the 60 first amino acids of said protein comprise a motif X1X2X3, wherein X1 is a Threonine (T), X2 is a Proline (P), and X3 is a Proline (P) or a Glutamine (Q), designated herein as TPP/Q motif,
- and wherein said dominant mutation comprises the mutation of at least one residue of the motif X1X2X3 of said TDM protein and results in the ability of the plant to produce Second Division Restitution 2n gametes.
Dependencies are inferred from the claim text.