US 2017/0254805 A1 · BIOCHEMICAL MOLECULE DETECTION SENSOR AND METHOD FOR DETECTING SPECIFIC MOLECULE USING MULTI-WAVELENGTH FLUORESCENCE

Abstract

A biochemical molecule detection sensor includes a substrate; a first aptamer complex chemically bound to the substrate, wherein the first aptamer complex selectively binds to a biochemical molecule, and includes at least one double helix DNA into which a fluorescent dye generating first fluorescence with a first wavelength is intercalated; and at least one second aptamer complex selectively binding to the biochemical molecule and generating second fluorescence with a second wavelength, wherein the first wavelength is different from the second wavelength, wherein fluorescence intensities of the first and second fluorescence depend on an amount of the biochemical molecule reacting with the first aptamer complex and the second aptamer complex.

Claims13

Dependent claims

A biochemical molecule detection sensor comprising:

a substrate;
a first aptamer complex chemically bound to the substrate, wherein the first aptamer complex selectively binds to a biochemical molecule, and includes at least one double helix DNA into which a fluorescent dye generating first fluorescence with a first wavelength is intercalated;
and
at least one second aptamer complex selectively binding to the biochemical molecule and generating second fluorescence with a second wavelength, wherein the first wavelength is different from the second wavelength,
wherein fluorescence intensities of the first and second fluorescence depend on an amount of the biochemical molecule reacting with the first aptamer complex and the second aptamer complex.

2 more elements

A method for detecting a biochemical molecule using multi-wavelength fluorescence, the method comprising:

applying a reagent onto a first aptamer complex, wherein the first aptamer complex is chemically bound to the substrate, wherein the first aptamer complex selectively binds to the biochemical molecule, and includes at least one double helix DNA into which a fluorescent dye generating first fluorescence with a first wavelength is intercalated, wherein the reagent includes the biochemical molecule and at least one second aptamer complex selectively binding to the biochemical molecule and generating second fluorescence with a second wavelength, wherein the first wavelength is different from the second wavelength;
removing the second aptamer complex not bound to the biochemical molecule;
and
measuring fluorescence intensities of the first and second fluorescence.

1 more element

Dependencies are inferred from the claim text.

Facts

Application

15/449,049Filed 3 Mar 2017

Priority

KR 10-2016-00254643 Mar 2016

Published

7 Sep 2017

Term ends

Not estimatedThe source records don’t support an estimate.

Classification7 codes

C12Q 1/68
Measuring or testing processes involving enzymes, nucleic acids or microorganisms ; Compositions therefor; Processes of preparing such…
C12Q 1/6813
Involving nucleic acids: hybridisation assays
C12Q 1/6816
Hybridisation assays: characterised by the detection means
G01N 33/5308
Immunoassay; Biospecific binding assay; Materials therefor: for analytes not provided for elsewhere, e.g. nucleic acids, uric acid, worms, mites

Biochemical molecule detection sensor and method for detecting specific molecule using multi-wavelength fluorescence

Life of this patent

Biochemical molecule detection sensor and method for detecting specific molecule using multi-wavelength fluorescence

Life of this patent

Abstract

Claims13