US 2017/0254803 A1 · METHOD FOR THE DETECTION OF ANTIGEN PRESENTATION

Abstract

The present invention pertains to a method for detecting antigen presentation via antigen presenting molecules such as major histocompatibility complex (MHC) class I or II. The invention deploys a first binding agent specific for the antigen epitope and a second binding agent specific for the antigen presenting molecule. The binding agents of the invention are coupled to proximity probes which upon antigen presentation elicit a detectable signal. The method of the invention allows detecting antigen presentation via MHC in-vitro and in a tissue sample in-situ. Thus the method of the present invention finds application as a new diagnostic tool, for example in the diagnosis of various diseases such as infectious diseases, immunological disorders, in particular autoimmune diseases, and proliferative disorders such as cancer.

Claims19

Dependent claims

A method for detecting antigen presentation of an epitope by an antigen presentation molecule, comprising

(a) Providing a first binding agent and a second binding agent, wherein the first binding agent is capable of specifically binding the epitope, and the second binding agent is capable of specifically binding the antigen presentation molecule;
wherein the first and the second binding agent are characterized in that spatial proximity of the first binding agent and the second binding agent induces a detectable signal,
(b) Providing an antigen presentation molecule, wherein the antigen presentation molecule is suspected of presenting the epitope,
(c) Providing the epitope,
(d) Bringing into contact the epitope, the antigen presentation molecule, the first binding agent and the second binding agent,
wherein the presence of the detectable signal is indicative for the antigen presentation of the epitope by the antigen presentation molecule.

3 more elements

Dependencies are inferred from the claim text.

Method for the detection of antigen presentation

Life of this patent

Method for the detection of antigen presentation

Life of this patent

Abstract

Claims19