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US 2017/0254801 A1Published application
Method for identifying substances which prime a defense response
AssigneeRheinisch WestfäLische Technische Hochschule Aachen (Rwth)
InventorsBritta SCHILLHEIM, Jochen BÜCHS, Uwe CONRATH, Jana SCHILLING
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Abstract
The present invention relates to a method for identifying substances which prime cells for a stress response by determining the respiration activity of the cells treated with a candidate substance in comparison to cells not treated with the candidate substance.
Claims16
A method for identifying substances which prime cells for a stress response comprising the steps of:
- inoculating cells in a suitable culture medium and culturing them in said medium;
- adding one or more candidate substances to the culture medium thereby treating the cells with said candidate substance;
- and
- measuring the respiration activity in the cells treated with the candidate substance and in control cells not treated with the candidate substance, wherein an increase in the respiration activity in the cells treated with the candidate substance compared to the control cells indicates that the candidate substance primes the cells for a stress response.
1 more element
A method for identifying substances which prime plant cells for a stress response comprising the steps of:
- inoculating plant cells in a suitable culture medium and culturing them in said medium;
- adding one or more candidate substances to the culture medium thereby treating the plant cells with said candidate substance;
- and
- measuring the oxygen transfer rate in the cells treated with the candidate substance and in control cells not treated with the candidate substance,
- wherein an increase in the oxygen transfer rate in the cells treated with the candidate substance
- compared to the control cells indicates that the candidate substance primes the plant cells for a stress response.
3 more elements
(canceled)
Dependencies are inferred from the claim text.