Methods and compositions for obtaining marker-free transgenic plants

A transgenic cell transformed with and comprising each of:

• (1) a first DNA segment comprising left and right T-DNA borders flanking a gene of interest operably linked to a promoter functional in plants and (2) a second DNA segment comprising a second set of left and right T-DNA borders flanking a promoter functional in a seed operably linked to a DNA cassette that confers a detectable phenotype in seeds of a plant comprising the DNA cassette;
• and a marker gene operably linked to a promoter functional in plants.

41-48. (canceled)

A composition comprising a mixture of (1) a first DNA segment comprising left and right T-DNA borders flanking a gene of interest operably linked to a promoter functional in plants;

• and (2) a second DNA segment comprising a second set of left and right T-DNA borders flanking a promoter functional in a seed operably linked to a DNA cassette that confers a detectable phenotype in seeds of a plant comprising the DNA cassette;
• and a marker gene operably linked to a promoter functional in plants.

A composition comprising bacterial cells, the composition comprising (1) a first DNA segment comprising left and right T-DNA borders flanking a gene of interest operably linked to a promoter functional in plants;

• and (2) a second DNA segment comprising a second set of left and right T-DNA borders flanking a promoter functional in a seed operably linked to a DNA cassette that confers a detectable phenotype in seeds of a plant comprising the DNA cassette;
• and a marker gene operably linked to a promoter functional in plants.