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US 2017/0253934 A1Published application
Method for screening induced pluripotent stem cells
AssigneeKyoto University
InventorsShinya YAMANAKA, Kazutoshi TAKAHASHI, Michiyo KOYANAGI, Mari OHNUKI
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Abstract
The present invention provides a method for screening for iPS cells exhibiting differentiation resistance using a marker identified as lincRNA or mRNA that is specifically expressed in an iPS cell line exhibiting differentiation resistance, and such markers.
Claims2
A method for screening for a human induced pluripotent stem cell line exhibiting no differentiation resistance, comprising the steps of:
- (i) measuring expression of at least one of HHLA1 mRNA and ABHD12B mRNA, and
- (ii) selecting a human induced pluripotent stem cell line in which the at least one of HHLA1 mRNA and ABHD12B mRNA does not express, and
- wherein said selected human induced pluripotent stem cell line exhibits no differentiation resistance.
A method for screening for a human induced pluripotent stem cell line exhibiting no differentiation resistance, comprising the steps of;
- (i) measuring expression of at least one of HHLA1 mRNA and ABHD12B mRNA, and
- (ii) selecting a human induced pluripotent stem cell line in which an expression level of at least one of HHLA1 mRNA and ABHD12B mRNA is equal to the expression level of a control ES cell line or a control induced pluripotent stem cell line known to exhibit no differentiation resistance, or lower than the expression level of an induced pluripotent stem cell line known to exhibit differentiation resistance, and
- wherein said selected human induced pluripotent stem cell line exhibits no differentiation resistance.
Dependencies are inferred from the claim text.