Method for cell recovery
AssigneeNissan Chemical Industries, Ltd.
InventorsAyako AIHARA (formerly OTANI), Taito NISHINO
Life of this patent
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Abstract
The present invention provides a method of isolating cells or tissues from a culture preparation of the cells or tissues in a medium composition which enables culture of the cells or tissues in suspension, which comprises at least one step selected from the group consisting of the following (A), (B) and (C): (A) passing the culture preparation through a filter having fine pores having a pore diameter of 5-500 μm, (B) adding a chelator to the culture preparation, and (C) diluting the culture preparation with a physiological aqueous solution.
Claims18
A method of isolating cells or tissues from a culture preparation of the cells or tissues in a medium composition which enables culture of the cells or tissues in suspension, which comprises at least one step selected from the group consisting of the following (A), (B) and (C):
- (A) passing the culture preparation through a filter having fine pores with a diameter of 5-500 μm,
- (B) adding a chelator to the culture preparation, and
- (C) diluting the culture preparation with a physiological aqueous solution.
-13. (canceled)
Dependencies are inferred from the claim text.