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AssigneesInstitut National de la Recherche Agronomique, Centre International D'Etudes Superieures En Sciences Agronomiques, Universite De Montpellier, Centre National De La Recherche Scientifique
InventorsPIERRE BERTHOMIEU, ORIANE MITH, LAURENCE MARQUES-BASTIDE, VÉRONIQUE PERRIER, ERIC DUBREUCQ
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The invention concerns a method for producing a compound of interest of the sterol biosynthesis pathway in a eukaryotic organism or a derivative of a compound of the sterol biosynthesis pathway in said eukaryotic organism, in particular a sterol. This method comprises a step for the in vitro culturing of cells of said eukaryotic organism modified to express or overexpress a defensin, in a culture medium suitable for the development of said cells and containing at least one element chosen from the transition metals, lead and selenium, at a concentration greater than or equal to the concentration of said element that is necessary for inducing the overproduction of said compound of interest by said cells.
A method for producing a compound of interest of a sterol biosynthesis pathway in a eukaryotic organism or derived from a compound of the sterol biosynthesis pathway in said eukaryotic organism, comprising a step of in vitro culture of cells of said eukaryotic organism, modified to express or overexpress a defensin, in an enriched culture medium configured to grow said cells and containing at least one element chosen from a group consisting of transition metals, lead and selenium, at a concentration greater than or equal to a concentration of said at least one element for inducing production by said cells of an amount of said compound of interest at least 2 times greater than an amount produced by said cells under identical culture conditions in a culture medium containing said at least one element at a minimum concentration for an optimal growth of said cells.
A recombinant eukaryotic cell modified to express or overexpress a defensin and modified to inhibit an enzyme activity involved in a biotransformation of an intermediate compound in a sterol biosynthesis pathway in said recombinant eukaryotic cell.
A method of using a recombinant yeast strain modified to express or overexpress a defensing for production of a compound of interest of a sterol biosynthesis pathway or derived from a compound of the sterol biosynthesis pathway in said recombinant yeast strain.
Dependencies are inferred from the claim text.