Novel anti-human tie-2 antibody

A polynucleotide selected from the group consisting of (a) and (b):

• (a) a polynucleotide comprising a base sequence encoding a heavy chain consisting of the amino acid sequence shown by SEQ ID NO:
• 2;
• and
• (b) a polynucleotide comprising a base sequence encoding a light chain consisting of the amino acid sequence shown by SEQ ID NO:
• 4.

A host cell selected from the group consisting of (a) to (d) below:

• (a) a host cell transformed with an expression vector comprising a polynucleotide comprising a base sequence encoding a heavy chain variable region consisting of the amino acid sequence of the amino acid numbers 1 to 122 of SEQ ID NO:
• 2 and a polynucleotide comprising a base sequence encoding a light chain variable region consisting of the amino acid sequence of the amino acid numbers 1 to 113 of SEQ ID NO:
• 4;
• (b) a host cell transformed with an expression vector comprising a polynucleotide comprising a base sequence encoding a heavy chain variable region consisting of the amino acid sequence of the amino acid numbers 1 to 122 of SEQ ID NO:
• 2 and an expression vector comprising a polynucleotide comprising a base sequence encoding a light chain variable region consisting of the amino acid sequence of the amino acid numbers 1 to 113 of SEQ ID NO:
• 4;
• (c) a host cell transformed with an expression vector comprising a polynucleotide comprising a base sequence encoding a heavy chain variable region consisting of the amino acid sequence of the amino acid numbers 1 to 122 of SEQ ID NO:
• 2;
• and
• (d) a host cell transformed with an expression vector comprising a polynucleotide comprising a base sequence encoding a light chain variable region consisting of the amino acid sequence of the amino acid numbers 1 to 113 of SEQ ID NO:
• 4.

A host cell selected from the group consisting of (a) to (d) below:

• (a) a host cell transformed with an expression vector comprising a polynucleotide comprising a base sequence encoding a heavy chain consisting of the amino acid sequence shown by SEQ ID NO:
• 2 and a polynucleotide comprising a base sequence encoding a light chain consisting of the amino acid sequence shown by SEQ ID NO:
• 4;
• (b) a host cell transformed with an expression vector comprising a polynucleotide comprising a base sequence encoding a heavy chain consisting of the amino acid sequence shown by SEQ ID NO:
• 2 and an expression vector comprising a polynucleotide comprising a base sequence encoding a light chain consisting of the amino acid sequence shown by SEQ ID NO:
• 4;
• (c) a host cell transformed with an expression vector comprising a polynucleotide comprising a base sequence encoding a heavy chain consisting of the amino acid sequence shown by SEQ ID NO:
• 2;
• and
• (d) a host cell transformed with an expression vector comprising a polynucleotide comprising a base sequence encoding a light chain consisting of the amino acid sequence shown by SEQ ID NO:
• 4.

A method for producing an anti-human Tie2 antibody or an antigen-binding fragment thereof, comprising culturing host cell(s) selected from the group consisting of (a) to (c) below to express a tetravalent anti-human Tie2 antibody or an antigen-binding fragment thereof:

• (a) a host cell transformed with an expression vector comprising a polynucleotide comprising a base sequence encoding a heavy chain variable region consisting of the amino acid sequence of the amino acid numbers 1 to 122 of SEQ ID NO:
• 2 and a polynucleotide comprising a base sequence encoding a light chain variable region consisting of the amino acid sequence of the amino acid numbers 1 to 113 of SEQ ID NO:
• 4;
• (b) a host cell transformed with an expression vector comprising a polynucleotide comprising a base sequence encoding a heavy chain variable region consisting of the amino acid sequence of the amino acid numbers 1 to 122 of SEQ ID NO:
• 2 and an expression vector comprising a polynucleotide comprising a base sequence encoding a light chain variable region consisting of the amino acid sequence of the amino acid numbers 1 to 113 of SEQ ID NO:
• 4;
• and
• (c) a host cell transformed with an expression vector comprising a polynucleotide comprising a base sequence encoding a heavy chain variable region consisting of the amino acid sequence of the amino acid numbers 1 to 122 of SEQ ID NO:
• 2 and a host cell transformed with an expression vector comprising a polynucleotide comprising a base sequence encoding a light chain variable region consisting of the amino acid sequence of the amino acid numbers 1 to 113 of SEQ ID NO:
• 4.

A method for producing an anti-human Tie2 antibody, comprising culturing host cell(s) selected from the group consisting of (a) to (c) below to express an anti-human Tie2 antibody:

• (a) a host cell transformed with an expression vector comprising a polynucleotide comprising a base sequence encoding a heavy chain consisting of the amino acid sequence shown by SEQ ID NO:
• 2 and a polynucleotide comprising a base sequence encoding a light chain consisting of the amino acid sequence shown by SEQ ID NO:
• 4;
• (b) a host cell transformed with an expression vector comprising a polynucleotide comprising a base sequence encoding a heavy chain consisting of the amino acid sequence shown by SEQ ID NO:
• 2 and an expression vector comprising a polynucleotide comprising a base sequence encoding the light chain consisting of the amino acid sequence shown by SEQ ID NO:
• 4;
• and
• (c) a host cell transformed with an expression vector comprising a polynucleotide comprising a base sequence encoding a heavy chain consisting of the amino acid sequence shown by SEQ ID NO:
• 2 and a host cell transformed with an expression vector comprising a polynucleotide comprising a base sequence encoding a light chain consisting of the amino acid sequence shown by SEQ ID NO:
• 4.