Cleavage resistant photoluminescent proteins and applications thereof
AssigneeBoard of Trustees of the University of Arkansas
InventorsRobert R. BEITLE, Rudra Palash Mukherjee, Suresh K. Thallapuranam, Joshua Sakon, David S. McNabb
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Abstract
Photoluminescent proteins resistant to protein cleavage reagents are described herein. In being resistant to cleavage reagents, such photoluminesncent proteins can reduce the number of digestion or cleavage products, thereby simplifying quantification and purification of recombinant protein compositions. In some embodiments, a photoluminescent protein described herein comprises an amino acid sequence having an absence of internal methionine residues.
Claims17
A photoluminescent protein comprising:
- an amino acid sequence having an absence of internal methionine residues.
A recombinant protein composition comprising:
- a first amino acid sequence of a target protein;
- and
- a photoluminescent protein coupled to the first amino acid sequence, the photoluminescent protein comprising an amino acid sequence having an absence of internal methionine residues.
A method of purifying recombinant protein composition comprising:
- providing a mixture including the recombinant protein composition, the recombinant protein composition comprising a first amino acid sequence of a target protein and a photoluminescent protein coupled to the first amino acid sequence, the photoluminescent protein including an amino acid sequence having an absence of internal methionine residues;
- and
- treating the recombinant protein composition with CNBr to release the recombinant protein from an intact photoluminescent protein.
Dependencies are inferred from the claim text.