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US 2017/0248620 A1Published application
Vitamin d metabolite determination utilizing mass spectrometry following derivatization
AssigneeQuest Diagnostics Investments Incorporated
InventorsBrett Holmquist, Nigel J. Clarke, Richard E Reitz
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Abstract
The invention relates to the detection of vitamin D metabolites. In a particular aspect, the invention relates to methods for detecting derivatized vitamin D metabolites by mass spectrometry.
Claims22
A method for determining an amount of one or more vitamin D metabolites in a sample by mass spectrometry, the method comprising:
- (i) adding one or more internal standards to a sample;
- (ii) extracting one or more vitamin D metabolites and the one or more internal standards from the sample;
- (iii) subjecting the one or more vitamin D metabolites and one or more internal standards to 4-phenyl-1,2,4-triazoline-3,5-dione (PTAD) derivatization;
- (iv) purifying by solid phase extraction (SPE);
- (v) ionizing the one or more PTAD-derivatized vitamin D metabolites derivatives and the one or more PTAD-derivatized internal standards;
- and
- (vi) determining an amount of the one or more PTAD-derivatized vitamin D metabolites and the one or more PTAD-derivatized internal standards by mass spectrometry
4 more elements
A method for determining an amount of one or more vitamin D metabolites in a sample by mass spectrometry, the method comprising:
- (i) adding one or more internal standards to a sample;
- (ii) extracting one or more vitamin D metabolites and the one or more internal standards from the sample;
- (iii) subjecting the one or more vitamin D metabolites and one or more internal standards to 4-phenyl-1,2,4-triazoline-3,5-dione (PTAD) derivatization;
- (iv) ionizing the one or more PTAD-derivatized vitamin D metabolites derivatives and the one or more PTAD-derivatized internal standards;
- and
- (v) determining an amount of the one or more PTAD-derivatized vitamin D metabolites and the one or more PTAD-derivatized internal standards by mass spectrometry
3 more elements
Dependencies are inferred from the claim text.