Bioavailability of Metal Ions
AssigneeColgate-Palmolive Company
InventorsLyndsay SCHAEFFER-KORBYLO, Betty WON, Venda Porter MALONEY, Gregory SZEWCZYK
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Abstract
Provided herein is a method for determining the bioavailability of a metal ion in a composition comprising a source of the metal ion, wherein the metal ion has antimicrobial activity, and wherein the method comprises: a) incubating a sample of the composition with bacterial cells, and b) determining the viability of the bacterial cells after incubation. The method is useful for determining the effect of agents on the bioavailability of metal ions in a composition.
Claims47
A method for determining the bioavailability of a metal ion in a composition comprising a source of the metal ion, wherein the metal ion has antimicrobial activity, and wherein the method comprises:
- a) incubating a sample of the composition comprising the source of the metal ion with bacterial cells, and
- b) determining the viability of the bacterial cells after incubation.
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A method of assessing the effect of an agent on the bioavailability of a metal ion in a composition comprising a source of the metal ion, wherein the metal ion has antimicrobial activity, and the method comprises:
- a) incubating a first sample of the composition comprising the source of the metal ion with bacterial cells,
- b) determining the viability of the bacterial cells after incubation,
- c) incorporating the agent into a second sample of the composition comprising the source of the metal ion,
- d) incubating the second sample of the composition comprising the source of the metal ion and the agent with bacterial cells,
- e) determining the viability of the bacterial cells after incubation, and
- f) comparing the viabilities as determined in steps b) and e).
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Dependencies are inferred from the claim text.