Method of detecting a salmonella microorganism

A method of detecting Salmonella microorganisms, the method comprising:

• providing,
• a sample to be tested;
• a culture device;
• a nutrient medium that facilitates growth of a Gram-negative enteric microorganism;
• a first selective agent that inhibits the growth of Gram-positive microorganisms;
• a first differential indicator system comprising a first differential indicator compound that can be converted to a first detectable product by a member of a group of Salmonella microorganisms that includes a microorganism of the species Salmonella bongori;
• and
• a second differential indicator system comprising a second differential indicator compound that can be converted to a second detectable product by a β-galactosidase enzyme activity;
• contacting in the culture device the nutrient medium, the first selective agent, the first differential indicator system, and the second differential indicator system with a sample to form an inoculated culture device;
• incubating the inoculated culture device for a first period of time at a temperature greater than 40 degrees C.;
• observing the culture device to detect a presence or an absence of the first detectable product;
• and
• observing the culture device to detect a presence or an absence of the second detectable product;
• wherein observing the presence of the first detectable product indicates a possible presence in the sample of a Salmonella microorganism;
• wherein observing the presence of the first detectable product juxtaposed with the second detectable product indicates a presence in the sample of a microorganism other than a β-galactosidase-producing member of the species Salmonella bongori.