Methods, devices, and systems for microfluidic stress emulation
AssigneesArizona Board of Regents on Behalf of the University of Arizona, The Research Foundation for The State University of New York
InventorsMarvin J. Slepian, Alberto C. Redaelli, Marco Rasponi, Danny Bluestein
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Abstract
Provided herein is technology relating to microfluidic devices and particularly, but not exclusively, to devices, methods, systems, and kits for imparting stresses on a fluid flowing through a microfluidic device that is designed to mimic a stress profile of a macrofluidic device or pathology.
Claims43
A method for monitoring thrombogenic state in subject, the method comprising:
- a) modeling the shear stress profile of a pathology or a medical device,
- wherein the pathology is a cardiovascular pathology,
- wherein the medical device is a mechanical circulatory support device,
- wherein the modeling comprises one or more of device thrombogenicity emulation, producing a probability density function of the medical device, and determining individual particle trajectories for particles flowing through the medical device;
- b) reproducing the shear stress profile of the pathology or device in a microfluidic device;
- c) flowing a sample from the subject through the microfluidic device, wherein the sample comprises platelets separated from a blood sampled obtained from the subject,
- wherein the sample has a volume of 1 to 100 nanoliters, 1 to 1000 nanoliters, 1 to 100 microliters, or 1 to 1000 microliters;
- and
- d) testing a thrombogenic potential of the sample to monitor a thrombogenic state of the subject wherein the testing a thrombogenic potential comprises testing a platelet activation state of the sample.
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A microfluidic device for imparting shear stress on a fluid sample that reproduces shear stress imparted on the fluid sample by a macrofluidic device or pathology
- wherein the device comprises a microfluidic channel comprising narrow regions to impart shear stress on the fluid sample flowing through the microfluidic channel,
- wherein the device comprises a channel designed to impart shear stress on a sample according to a shear stress history probability density function describing the macrofluidic device, a platelet separating component, a platelet activation state assay component, and a detector,
- wherein the device comprises one or more of a cell separation component, an assay component,
- wherein the shear stress imparted on the fluid sample by a macrofluidic device is modeled using device thrombogenicity emulation.
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A system for treating a patient having a cardiovascular disease, the system comprising:
- a) a macrofluidic device that contacts the cardiovascular system of the patient;
- and
- b) a microfluidic device that reproduces the shear stress imparted on blood by the macrofluidic device.
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Dependencies are inferred from the claim text.