Compositions and methods for detecting enterovirus d68
AssigneeThe United States of America, as represented by the Secretary, Department of Health and Human Serv
InventorsWilliam Allan Nix, M. Steven Oberste
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AssigneeThe United States of America, as represented by the Secretary, Department of Health and Human Serv
InventorsWilliam Allan Nix, M. Steven Oberste
Estimating the term…
Disclosed herein are methods and compositions for detection of enterovirus D in a sample, particularly detection of enterovirus D68. The methods include contacting a sample with at least one primer (such as a forward primer and/or a reverse primer) capable of specifically amplifying an EV-D68 viral protein 1 (VP1) nucleic acid or a portion thereof and/or a detectably labeled probe capable of specifically hybridizing to an EV-D68 VP1 nucleic acid, under conditions sufficient for specific amplification of the EV-D68 VP1 nucleic acid by the at least one primer and/or under conditions sufficient for specific hybridization of the probe to the EV-D68 nucleic acid. The amplification of the EV-D68 VP1 nucleic acid and/or the hybridization of the probe to the EV-D68 VP1 nucleic acid is detected, thereby identifying presence of EV-D68 in the sample.
A method of detecting presence of human enterovirus D68 (EV-D68) in a sample, comprising:
An isolated probe comprising a nucleic acid sequence at least 90% identical to GTCCATTTGAAAAAGTTCTTGTC (SEQ ID NO:
A kit for detecting human enterovirus D68 in a sample, comprising:
Dependencies are inferred from the claim text.