Endpoint Zygosity Assay To Detect RF4 Gene In Maize

A PCR assay method for determining zygosity of an Rf4 gene in a corn plant, the method comprising:

• performing a first PCR assay using a first probe, a first forward primer, and a first reverse primer on a polynucleotide from a corn plant sample;
• performing a second PCR assay using a second probe, a second forward primer, and a second reverse primer on the polynucleotide sample;
• quantifying the first and second probe;
• and,
• comparing the quantified first and second probe to determine zygosity.