Assay devices and assay assemblies

An assay assembly comprising a main housing and a capture device held inside the main housing, wherein the capture device is for capturing one specific biomolecule or a plurality of specific biomolecule and has a detection surface, the main housing comprises an analyte inlet, an analyte outlet and a hybridization compartment, and the capture device is held inside the hybridization compartment with the detection surface fully extended inside the hybridization compartment;

• wherein the analyte inlet is connected to an inlet end of the hybridization compartment by an analyte inlet channel and the analyte outlet is connected to an outlet end of the hybridization compartment by an analyte outlet channel;
• and wherein the hybridization compartment and the analyte inlet channel define a closed liquid passage path such that a liquid analyte entering the assay assembly through the analyte inlet and under a moving force or pressure will be guided to move along the analyte inlet channel, and to move through or across the hybridization compartment until subsequently discharged at the outlet end of the hybridization compartment and ultimately at the analyte outlet.