US 2016/0355875 A1 · METHODS AND KITS FOR DETECTING NUCLEIC ACID SEQUENCES OF INTEREST USING DNA-BINDING PROTEIN DOMAIN

Abstract

The present invention relates to a method for the detection of a specific nucleic acid. Specifically, the invention provides a method and kits for detecting the presence of a specific nucleic acid using engineered DNA-binding domains such as Transcription Activator Like-Effector (TALE) domain or modular base-per-base binding domains (MBBBD). The method of the invention is particularly useful for in vitro diagnostic application.

Claims16

Dependent claims

A method of detecting a nucleic acid sequence of interest comprising:

(a) Providing nucleic acids which can comprise the nucleic acid sequence of interest;
(b) Providing at least one DNA binding domain capable of binding said nucleic acid sequence of interest;
(c) Contacting said nucleic acids with said DNA binding domain;
(d) Removing unbound said DNA binding domain and nucleic acids and;
(e) Detecting the presence or the absence of the nucleic acid sequence of interest.

2 more elements

Dependencies are inferred from the claim text.

Facts

Application

15/028,644Filed 10 Oct 2014

Priority

DK PA 2013 7057811 Oct 2013

Published

8 Dec 2016

Term ends

Not estimatedThe source records don’t support an estimate.

Classification7 codes

C12Q 1/6816
Hybridisation assays: characterised by the detection means
C12Q 1/6806
Involving nucleic acids: preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
C12Q 1/6827
Hybridisation assays: for detection of mutation or polymorphism
C12Q 1/6841
Hybridisation assays: in situ hybridisation

Methods and kits for detecting nucleic acid sequences of interest using dna-binding protein domain

Life of this patent

Methods and kits for detecting nucleic acid sequences of interest using dna-binding protein domain

Life of this patent

Abstract

Claims16