Genetic markers for diagnosis of tuberculosis caused by mycobacterium tuberculosis
AssigneesEuropean Molecular Biology Laboratory, Kusuma School of Biological Sciences Indian Institute of Technology
InventorsSyed Asad RAHMAN, Javeed AHMAD, Nasreen Z EHTESHAM, Seyed E HASNAIN
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Abstract
The application is related to novel signature sequences for diagnosis of Mycobacterium tuberculosis in clinical samples with 100% specificity and a very high degree of sensitivity.
Claims6
A nucleotide sequence capable of selectively detecting pathogenic Mycobacterium sps using oligonucleotide primers corresponding to the signature sequence selected from SEQ ID NO:
- 1, 2, 3 or 4.
A method of detecting pathogenic mycobacterium sps in a clinical sample, said method comprising the steps of:
- a. removal of contaminants from the clinical sample by conventional methods;
- b. extraction of genomic DNA from the contaminant-free clinical sample;
- c. designing a set of specific oligonucleotide primers capable of specifically detecting SEQ ID NO:
- 1, 2, 3 or 4 for use in RT-PCR;
- d. analyzing PCR product by electrophoresis or specific probe nucleotide sequence complementary to SEQ ID NO:
- 1, 2, 3 or 4.
A kit for detection of pathogenic mycobacterium sps in clinical samples, said kit comprising set of oligonucleotide primers selected from
- (i)
- 5′ ATGCAGGTTGCGACTGTACACCCGG 3′
- 3′ GGCCGCTCTTGTTCTTCGTCGGAT 5′
- (ii)
- 5′ GTGTTTGCGTTGAGTAATAATCTGAACCGTGT 3′
- 3′ AGCCAATTCCAGCACGATGTCGCC 5′
- (iii)
- 5′ ATGCAGGTTGCGACTGTACACCCGG 3′
- 3′ GGCCGCTCTTGTTCTTCGTCGGAT 5′
- (iv)
- 5′ TGTACCGCGTGCCCGACGATTTG 3′
- 3′ ACAGGCAGCTAACAGGGCGTCGG 5′
- (v) a set of oligonucleotide primers complementary to (i), (ii), (iii) or (iv) or
- (vi) a set of oligonucleotide primers comprising of sequence containing any 10 consecutive bases from one of the sequences selected from SEQ ID NO:
- 1, 2, 3 or 4.
Dependencies are inferred from the claim text.