Rna polymerase ii33 nucleic acid molecules to control insect pests

A method for controlling a coleopteran or hemipteran pest population, the method comprising providing an agent comprising a ribonucleic acid (RNA) molecule that functions upon contact with the pest to inhibit a biological function within the pest, wherein the RNA is specifically hybridizable with a polynucleotide selected from the group consisting of any of SEQ ID NOs:92-102;

• the complement of any of SEQ ID NOs:92-102;
• a fragment of at least 15 contiguous nucleotides of any of SEQ ID NOs:92-102;
• the complement of a fragment of at least 15 contiguous nucleotides of any of SEQ ID NOs:92-102;
• a transcript of any of SEQ ID NOs:1, 3, 5-8, 76, 78, and 80-82;
• the complement of a transcript of any of SEQ ID NOs:1, 3, 5-8, 76, 78, and 80-82;
• a fragment of at least 15 contiguous nucleotides of a transcript of any of SEQ ID NOs:1, 3, 5-8, 76, 78, and 80-82;
• and the complement of a fragment of at least 15 contiguous nucleotides of a transcript of any of SEQ ID NOs:1, 3, 5-8, 76, 78, and 80-82.

A method of controlling coleopteran pest infestation in a plant, the method comprising providing in the diet of a coleopteran pest a ribonucleic acid (RNA) that is specifically hybridizable with a polynucleotide selected from the group consisting of:

• SEQ ID NOs:92-97;
• the complement of any of SEQ ID NOs:92-97;
• a fragment of at least 15 contiguous nucleotides of any of SEQ ID NOs:92-97;
• the complement of a fragment of at least 15 contiguous nucleotides of any of SEQ ID NOs:92-97;
• a transcript of any of SEQ ID NOs:1, 3, and 5-8;
• the complement of a transcript of any of SEQ ID NOs:1, 3, and 5-8;
• a fragment of at least 15 contiguous nucleotides of a transcript of SEQ ID NO:1 or SEQ ID NO:3;
• and
• the complement of a fragment of at least 15 contiguous nucleotides of a transcript of SEQ ID NO:1 or SEQ ID NO:3.

A method of controlling hemipteran pest infestation in a plant, the method comprising contacting a hemipteran pest with a ribonucleic acid (RNA) that is specifically hybridizable with a polynucleotide selected from the group consisting of:

• SEQ ID NOs:98-102;
• the complement of any of SEQ ID NOs:98-102;
• a fragment of at least 15 contiguous nucleotides of any of SEQ ID NOs:98-102;
• the complement of a fragment of at least 15 contiguous nucleotides of any of SEQ ID NOs:98-102;
• a transcript of any of SEQ ID NOs:76, 78, and 80-82;
• the complement of a transcript of any of SEQ ID NOs:76, 78, and 80-82;
• a fragment of at least 15 contiguous nucleotides of a transcript of SEQ ID NO:76 or SEQ ID NO:78;
• and
• the complement of a fragment of at least 15 contiguous nucleotides of a transcript of SEQ ID NO:76 or SEQ ID NO:78.

A method for producing a transgenic plant cell, the method comprising:

• transforming a plant cell with a vector comprising a means for providing coleopteran pest protection to a plant;
• culturing the transformed plant cell under conditions sufficient to allow for development of a plant cell culture comprising a plurality of transformed plant cells;
• selecting for transformed plant cells that have integrated the means for providing coleopteran pest protection to a plant into their genomes;
• screening the transformed plant cells for expression of a means for inhibiting expression of an essential gene in a coleopteran pest;
• and
• selecting a plant cell that expresses the means for inhibiting expression of an essential gene in a coleopteran pest.

A method for producing a transgenic plant cell, the method comprising:

• transforming a plant cell with a vector comprising a means for providing hemipteran pest protection to a plant;
• culturing the transformed plant cell under conditions sufficient to allow for development of a plant cell culture comprising a plurality of transformed plant cells;
• selecting for transformed plant cells that have integrated the means for providing hemipteran pest protection to a plant into their genomes;
• screening the transformed plant cells for expression of a means for inhibiting expression of an essential gene in a hemipteran pest;
• and
• selecting a plant cell that expresses the means for inhibiting expression of an essential gene in a hemipteran pest.