Methods of identifying and selecting maize plants with resistance to anthracnose stalk rot
AssigneesE. I. Du Pont de Nemours and Company, Pioneer Hi-Bred International, Inc.
InventorsSCOTT B DAVIS, Jacso Dellai, Mark Timothy Jung, Ana Beatriz Locatelli, Petra J Wolters
Life of this patent
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Abstract
Compositions and methods useful in identifying and/or selecting maize plants that have anthracnose stalk rot resistance are provided herein. The resistance may be newly conferred or enhanced relative to a control plant. The methods use maize markers on chromosome 10 to identify, select and/or construct resistant plants. Maize plants generated by the methods also provided.
Claims15
A method of identifying and/or selecting a maize plant with anthracnose stalk rot resistance, said method comprising:
- a. analyzing DNA of a maize plant for the presence of a QTL allele on chromosome 10 that is associated with anthracnose stalk rot resistance, wherein said QTL allele comprises:
- i. a “T” at sbd_INBREDA_4,
- ii. a “C” at sbd_INBREDA_9,
- iii. a “T” at sbd_INBREDA_13,
- iv. a “T” at sbd_INBREDA_24,
- v. a “T” at sbd_INBREDA_25,
- vi. a “C” at sbd_INBREDA_32,
- vii. an “A” at sbd_INBREDA_33, and
- viii. a “G” at sbd_INBREDA_35;
- b. selecting said maize plant as having anthracnose stalk rot resistance if said QTL allele is detected;
- c. crossing the maize plant selected in (b) with a second maize plant;
- and
- d. obtaining a progeny plant that has the QTL allele.
A method of identifying and/or selecting a maize plant with anthracnose stalk rot resistance, said method comprising:
- a. detecting in a maize plant at least one marker allele that is linked to and associated with one or more marker alleles selected from the group consisting of:
- i. a “T” at sbd_INBREDA_4,
- ii. a “C” at sbd_INBREDA_9,
- iii. a “T” at sbd_INBREDA_13,
- iv. a “T” at sbd_INBREDA_24,
- v. a “T” at sbd_INBREDA_25,
- vi. a “C” at sbd_INBREDA_32,
- vii. an “A” at sbd_INBREDA_33, and
- viii. a “G” at sbd_INBREDA_35;
- b. selecting said maize plant that has the at least one marker allele that is linked to and associated with one or more marker alleles set forth in (i)-(viii) of step (a);
- c. crossing the maize plant selected in (b) with a second maize plant;
- and
- d. obtaining a progeny plant that has the at least one marker allele that is linked to and associated with one or more marker alleles set forth in (i)-(viii) of step (a).
A method of identifying and/or selecting a maize plant with anthracnose stalk rot resistance, said method comprising:
- a. detecting in a maize plant at least one marker allele that is linked to and associated with a haplotype comprising:
- i. a “T” at sbd_INBREDA_4,
- ii. a “C” at sbd_INBREDA_9,
- iii. a “T” at sbd_INBREDA_13,
- iv. a “T” at sbd_INBREDA_24,
- v. a “T” at sbd_INBREDA_25,
- vi. a “C” at sbd_INBREDA_32,
- vii. an “A” at sbd_INBREDA_33, and
- viii. a “G” at sbd_INBREDA_35;
- b. selecting said maize plant that has the at least one marker allele that is linked to and associated with the haplotype;
- c. crossing the maize plant selected in (b) with a second maize plant;
- and
- d. obtaining a progeny plant that has at least one marker allele that is linked to and associated with the haplotype in (a).
A method of identifying and/or selecting a maize plant with anthracnose stalk rot resistance, said method comprising:
- a. detecting in the maize plant a haplotype comprising:
- i. a “T” at sbd_INBREDA_4,
- ii. a “C” at sbd_INBREDA_9,
- iii. a “T” at sbd_INBREDA_13,
- iv. a “T” at sbd_INBREDA_24,
- v. a “T” at sbd_INBREDA_25,
- vi. a “C” at sbd_INBREDA_32,
- vii. an “A” at sbd_INBREDA_33, and
- v. a “G” at sbd_INBREDA_35;
- b. selecting said maize plant that has said haplotype;
- c. crossing the maize plant selected in (b) with a second maize plant;
- and
- d. obtaining a progeny plant that has the haplotype in (a).
A method of introgressing a QTL allele associated with anthracnose stalk rot resistance into a maize plant said method comprising:
- a. screening a population with at least one marker to determine if one or more maize plants from the population comprises a QTL allele associated with anthracnose stalk rot resistance, wherein the QTL allele comprises:
- i. a “T” at sbd_INBREDA_4,
- ii. a “C” at sbd_INBREDA_9,
- iii. a “T” at sbd_INBREDA_13,
- iv. a “T” at sbd_INBREDA_24,
- v. a “T” at sbd_INBREDA_25,
- vi. a “C” at sbd_INBREDA_32,
- vii. an “A” at sbd_INBREDA_33, and
- viii. a “G” at sbd_INBREDA_35;
- b. selecting from said population at least one maize plant comprising the QTL allele;
- c. crossing the maize plant selected in (b) with a second maize plant;
- and
- d. obtaining a progeny plant that comprises the QTL allele.
Dependencies are inferred from the claim text.