Polynucleotides and polypeptides involved in post-transcriptional gene silencing
AssigneeE. I. Du Pont de Nemours and Company
InventorsRebecca E. Cahoon, HAJIME SAKAI, J ANTONI RAFALSKI
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Abstract
This invention relates to isolated nucleic acid fragments encoding polypeptides involved in post-transcriptional gene silencing. The invention also relates to construction of a recombinant DNA construct encoding all or a portion of the polypeptide involved in post-transcriptional gene silencing, in sense or antisense orientation, wherein expression of the recombinant DNA construct results in production of altered levels in a transformed host cell of the the polypeptide involved in post-transcriptional gene silencing.
Claims14
A recombinant DNA construct comprising a polynucleotide operably linked to at least one heterologous regulatory sequence, said polynucleotide comprising:
- (a) a nucleotide sequence encoding a polypeptide, wherein the amino acid sequence of the polypeptide has at least 95% sequence identity to the amino acid sequence of SEQ ID NO:
- 2, or
- (b) the complement of the nucleotide sequence of (a),
- wherein a transgenic plant expressing the polynucleotide has an altered level of transposon silencing compared to a non-transgenic plant.
A cDNA comprising a nucleotide sequence encoding a polypeptide having at least 95% sequence identity to the amino acid sequence of SEQ ID NO:
- 2.
Dependencies are inferred from the claim text.