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US 2016/0355832 A1Published application
Novel method for the production of transgenic plants
AssigneeMonsanto Technology LLC
InventorsYinghui Dan, Joyce E. Fry
Life of this patent
Estimating the term…
Abstract
Mesocotyl meristem explants that contain multiple primary meristems are transformed via particle bombardment or Agrobacterium-mediated methods. Regeneration is through an organogenesis pathway that allows for secondary multiple bud formation. This method allows for the genotype independent transformation of varieties of wheat.
Claims21
1-16. (canceled)
A method of producing a transgenic monocot plant comprising:
- providing an explant of a monocot plant presenting a plurality of meristems;
- culturing said explant in a first multiple bud inducing media suitable for inducing production of a plurality of buds from at least one of said meristems;
- introducing exogenous DNA into at least one of said plurality of buds;
- removing said plurality of buds from said first media and transferring said plurality of buds to a second media suitable for induction of elongation of said buds into shoots;
- harvesting and transferring said shoots to a culture medium that promotes root development;
- and
- culturing said transferred shoots to produce plants.
4 more elements
A method of producing a transgenic monocot plant comprising:
- providing a monocot mesocotyl explant presenting a plurality of meristems;
- culturing said mesocotyl explant on a first media, comprising thidiazuron at a concentration of between about 2.0 mg/L and 7.5 mg/L, and 2,4-D at a concentration of about 0.5 mg/L and 2.0 mg/L, to induce the production of a plurality of buds from at least one of said plurality of meristems;
- introducing exogenous DNA into at least one of the cells of said plurality of buds;
- removing said plurality of buds from said first media and transferring said plurality of buds to a second media suitable for induction of elongation of said buds into shoots;
- culturing said shoots to produce plants.
2 more elements
Dependencies are inferred from the claim text.