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US 2016/0355829 A1Published application
Recombinant microorganism for improved production of fine chemicals
AssigneeBASF SE
InventorsHartwig SCHRÖDER, Holger HARTMANN, Qingzhao WANG, Shakir RATANI, Zheyuan GUO, Markus POMPEJUS
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Abstract
The present invention relates to a recombinant nucleic acid molecule, a recombinant microorganism, to a method for producing alanine and to the use of the recombinant nucleic acid molecule or the recombinant microorganism for the fermentative production of alanine.
Claims22
A recombinant nucleic acid molecule having a sequence selected from the group of
- (I) a nucleic acid molecule comprising a sequence of SEQ ID NO:
- 1, 45 or 49 or
- (II) a nucleic acid molecule having at least 80% identity to a nucleic acid molecule having a sequence of SEQ ID NO:
- 1, 45 or 49 or
- (III) a nucleic acid molecule hybridizing to the complement of a nucleic acid molecule having a sequence of SEQ ID NO:
- 1, 45 or 49 under stringent conditions, or
- (IV) a nucleic acid molecule encoding a polypeptide having a sequence of SEQ ID NO:
- 2, 46, or 50 or
- (V) a nucleic acid molecule encoding a polypeptide having at least 60% homology to a polypeptide having a sequence of SEQ ID NO:
- 2, 46 or 50
- wherein the codon of the genes under (I) to (V) corresponding to position 13-15 of SEQ ID NO:
- 1 is not encoding amino acid glutamine and is not a stop codon or the amino acid of the proteins encoded by the genes under (I) to (V) corresponding to position 5 of SEQ ID NO:
- 2 is not glutamine and is not a stop codon, and
- wherein the codon of the genes under (I) to (V) corresponding to position 913-915 of SEQ ID NO:
- 45 is not encoding amino acid arginine and is not a stop codon or the amino acid of the proteins encoded by the genes under (I) to (V) corresponding to position 305 of SEQ ID NO:
- 46 is not arginine and is not a stop codon, and
- wherein the codon of the genes under (I) to (V) corresponding to position 208-210 of SEQ ID NO:
- 49 is not encoding amino acid alanine and is not a stop codon or the amino acid of the proteins encoded by the genes under (I) to (V) corresponding to position 70 of SEQ ID NO:
- 50 is not alanine and is not a stop codon.
16 more elements
A recombinant amino acid molecule having a sequence selected from the group of
- (VI) an amino acid molecule comprising a sequence of SEQ ID NO:
- 2, or
- (VII) an amino acid molecule having at least 60% identity to a nucleic acid molecule having a sequence of SEQ ID NO:
- 2, or
- wherein the amino acid of the protein under (VII) corresponding to position 5 of SEQ ID NO:
- 2 is not glutamine.
3 more elements
A recombinant amino acid molecule having a sequence selected from the group of
- (VI) an amino acid molecule comprising a sequence of SEQ ID NO:
- 46, or
- (VII) an amino acid molecule having at least 60% identity to a nucleic acid molecule having a sequence of SEQ ID NO:
- 46, or
- wherein the amino acid of the protein under (VII) corresponding to position 305 of SEQ ID NO:
- 46 is not arginine.
3 more elements
A recombinant amino acid molecule having a sequence selected from the group of
- (VI) an amino acid molecule comprising a sequence of SEQ ID NO:
- 50, or
- (VII) an amino acid molecule having at least 60% identity to a nucleic acid molecule having a sequence of SEQ ID NO:
- 50, or
- wherein the amino acid of the protein under (VII) corresponding to position 70 of SEQ ID NO:
- 50 is not alanine.
3 more elements
A recombinant microorganism comprising at least one of
- a) an introduced, increased, enhanced or altered activity and/or expression of a lpd gene or a homolog or functional variant thereof,
- b) an introduced, increased, enhanced or altered activity and/or expression of a zipA gene or a homolog or functional variant thereof,
- c) (I) a nucleic acid molecule comprising a sequence of SEQ ID NO:
- 1 or
- (II) a nucleic acid molecule having at least 80% identity to a nucleic acid molecule having a sequence of SEQ ID NO:
- 1 or
- (III) a nucleic acid molecule hybridizing to the complement of a nucleic acid molecule having a sequence of SEQ ID NO:
- 1 under stringent conditions, or
- (IV) a nucleic acid molecule encoding a polypeptide having a sequence of SEQ ID NO:
- 2 or
- (V) a nucleic acid molecule encoding a polypeptide having at least 60% homology to a polypeptide having a sequence of SEQ ID NO:
- 2
- wherein the codon of the genes under c) (I) to (V) corresponding to position 13-15 of SEQ ID NO:
- 1 is not encoding amino acid glutamine and is not a stop codon or the amino acid of the proteins encoded by the genes under c) (I) to (V) corresponding to position 5 of SEQ ID NO:
- 2 is not glutamine.
12 more elements
16-30. (canceled)
32-35. (canceled)
37-41. (canceled)
43-50. (canceled)
Dependencies are inferred from the claim text.