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AssigneeYale University
InventorsFarren J. Isaacs, Miriam Amiram, Adrian Haimovich, Dieter Söll
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Compositions, systems, and methods for preparation of polypeptides having multiple iterations of non-standard amino acids are provided. The compositions and method can be used to produce recombinant proteins at a greater yield than the same or similar polypeptides made using conventional compositions, systems, and methods. Accordingly, in some embodiments, the polypeptides are ones that could not be made using conventional methods and reagents, or could not be made a sufficient yield or purity to serve a practical purpose using conventional methods and reagents. Polypeptides made using the disclosed compositions, systems, and methods are also provided.
A method of making aminoacyl tRNA synthetases (AARS) variants comprising
An isolated AARS variant comprising at least 90% sequence identity, but not 100% sequence identity, to the non-standard amino acid (amino acid ligand) binding pocket of any of SEQ ID NOS:2-15, the tRNA anticodon recognition interface of any of SEQ ID NO:2-15, or a combination thereof, or SEQ ID NO:1.
An isolated variant AARS comprising the non-standard amino acid (amino acid ligand) binding pocket of any of SEQ ID NOS:2-15, the tRNA anticodon recognition interface of any of SEQ ID NO:2-15, or a combination thereof.
A polypeptide comprising the amino acid sequence of any of SEQ ID NOS:20-27.
A polypeptide comprising the amino acid sequence of any of SEQ ID NOS:19-27, wherein “X” is 3,4-dihydroxyphenylalanine.
A polypeptide comprising the amino acid sequence of SEQ ID NOS:19-27 wherein “X” is pAzF and the side chain of pAzF is conjugated to a molecule.
The polypeptide of claim 33, wherein molecule is a fatty acid.
Dependencies are inferred from the claim text.