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US 2016/0355799 A1Published application
Polypeptides having cellulase activity
AssigneeCalifornia Institute of Technology
InventorsFrances H. Arnold, Pete Heinzelman
Life of this patent
Estimating the term…
Abstract
The present disclosure relates to CBH II chimera fusion polypeptides, nucleic acids encoding the polypeptides, and host cells for producing the polypeptides.
Claims12
(canceled)
A recombinant polypeptide selected from the group consisting of a, polypeptide comprising a sequence that is (a) identical to SEQ ID NO:67 and having a C400S mutation;
- (b) is at least 85% identical to SEQ ID NO:68 and having a C400S mutation;
- (c) is at least 85% identical to SEQ ID NO:69 and having a C400S mutation;
- (d) is at least 85% identical to SEQ ID NO:70 and having a C400S mutation;
- (e) is at least 85% identical to SEQ ID NO:71 and having a C400S mutation;
- (f) is at least 85% identical to SEQ ID NO:72 and having a C400S mutation;
- (d) is at least 85% identical to SEQ ID NO:73 and having a C400S mutation;
- (h) is at least 85% identical to SEQ ID NO:74 and having a C400S mutation;
- (i) is at least 85% identical to SEQ ID NO:75 and having a C400S mutation;
- (j) is at least 85% identical to SEQ ID NO:76 and having a C407S mutation;
- (k) is at least 85% identical to SEQ ID NO:77 and having a C394S mutation;
- and (l) is at least 85% identical to SEQ ID NO:78 and having a C412S, wherein the foregoing polypeptides have cellulase activity and improved thermostability compared to their corresponding parental (wild-type) protein lacking a Cys→Ser mutation.
8 more elements
4-7. (canceled)
Dependencies are inferred from the claim text.