Flow-through paramagnetic particle-based cell separation and paramagnetic particle removal
AssigneesNovartis AG, The Trustees of the University of Pennsylvania
InventorsFabio Fachin, Rodney Rietze, Michael R. Greene, Lan Cao
Life of this patent
Estimating the term…
Abstract
The present disclosure relates to systems and methods for flow-through separation of paramagnetic particle-bound cells in a cell suspension containing both bound and unbound cells as well as systems and methods for removing paramagnetic particles from paramagnetic particle-bound cells or from a cell suspension with unbound cells. It further relates to a flow-through magnetic separation/debeading module and a flow-through spinning membrane debeading module.
Claims47
A cell processing system comprising:
- at least one cell suspension module;
- at least one buffer module;
- at least one flow-through magnetic separation/debeading module;
- at least one non-magnetic output module;
- and
- at least one magnetic output module.
A flow-through magnetic separation/debeading module comprising:
- a chamber defined by walls and having an x-direction, a y-direction, and a z-direction;
- an inlet and an outlet arranged on opposite ends of the chamber in the y-direction;
- and
- at least two magnets adjacent or proximate a wall of the chamber and arranged to establish a zero gradient line within the chamber between the inlet and the outlet.
A spinning membrane debeading module comprising:
- a debeading chamber define partially by a cylindrical side-wall;
- a porous spinning membrane having an interior and oriented co-axially with the cylindrical side-wall;
- a sample inlet;
- a waste output module connected to the interior of the spinning membrane;
- a cell output module connected to the debeading chamber;
- and
- at least one magnet adjacent or proximate to the cylindrical side-wall.
A method of flow-through cell processing comprising flowing a cell suspension comprising paramagnetic particle-bound cells through a flow-through magnetic separation/debeading module to produce an unbound cell product,
- wherein the paramagnetic particle-bound cells continue to move in the flow-through magnetic separation/debeading module through the flowing step, and
- wherein the flow-through magnetic separation/debeading module comprises:
- a flow chamber defined by walls through which the cell suspension flows;
- and
- at least two magnets arranged adjacent or proximate at least one wall.
Dependencies are inferred from the claim text.