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US 2016/0355776 A1Published application
Bioreactor using acoustic standing waves
AssigneeFloDesign Sonics, Inc.
InventorsBart Lipkens, Louis Masi, Stanley Kowalski, III, Walter M. Presz, JR., Jason Dionne, Brian Dutra, Ari Mercado, Thomas J. Kennedy, III, Arthur Martin
Life of this patent
Estimating the term…
Abstract
A perfusion bioreactor includes at least one ultrasonic transducer that can acoustically generate a multi-dimensional standing wave. The standing wave can be used to retain cells in the bioreactor, and can also be utilized to dewater or further harvest product from the waste materials produced in a bioreactor.
Claims20
A method for obtaining expanded cells, comprising:
- culturing cells in a system comprising:
- a bioreactor including a reaction vessel having an internal volume, an agitator, a feed inlet, and an outlet;
- and
- a filtering device, comprising:
- an inlet fluidly connected to the bioreactor outlet;
- a flow chamber;
- an ultrasonic transducer and a reflector located opposite the ultrasonic transducer, the ultrasonic transducer being driven to produce a multi-dimensional acoustic standing wave in the flow chamber;
- and
- a recycle outlet downstream of the flow chamber connected to a recycle inlet of the reaction vessel;
- wherein the cells are cultured in a host fluid in the internal volume of the bioreactor to expand the cells;
- sending a portion of the host fluid containing the cells from the bioreactor to the flow chamber;
- trapping cells from the host fluid in the multi-dimensional acoustic standing wave;
- wherein cells that are trapped in the multi-dimensional acoustic standing wave form cell clusters that grow to a critical size and subsequently fall out of the multi-dimensional acoustic standing wave.
10 more elements
A method for culturing viruses or exosomes, comprising:
- culturing cells in a system comprising:
- a bioreactor including a reaction vessel having an internal volume, an agitator, a feed inlet, and an outlet;
- and
- a filtering device, comprising:
- an inlet fluidly connected to the bioreactor outlet;
- a flow chamber;
- an ultrasonic transducer and a reflector located opposite the ultrasonic transducer, the ultrasonic transducer being driven to produce a multi-dimensional acoustic standing wave in the flow chamber;
- a product outlet;
- and
- a recycle outlet downstream of the flow chamber connected to a recycle inlet of the reaction vessel;
- wherein the cells are cultured in a host fluid in the internal volume of the bioreactor to expand the cells, and wherein the cells are engineered to produce the viruses or exosomes;
- continuously sending a portion of the host fluid from the bioreactor to the flow chamber;
- trapping the viruses or exosomes in the multi-dimensional acoustic standing wave;
- wherein the viruses or exosomes that are trapped in the multi-dimensional acoustic standing wave form clusters that grow to a critical size and subsequently fall out of the multi-dimensional acoustic standing wave into the product outlet;
- and
- recovering the viruses or exosomes from the product outlet.
13 more elements
Dependencies are inferred from the claim text.