Novel bispecific antibody binding to human tlr2 and human tlr4

A method for producing a bispecific antibody binding to human TLR2 and human TLR4, comprising culturing host cell(s) selected from the group consisting of the following (a) to (c) to express the bispecific antibody binding to human TLR2 and human TLR4:

• (a) a host cell transformed with an expression vector comprising a polynucleotide comprising a base sequence encoding a heavy chain variable region of an anti-human TLR2 antibody consisting of the amino acid sequence of amino acid numbers 1 to 120 of SEQ ID NO:
• 4, a polynucleotide comprising a base sequence encoding a heavy chain variable region of an anti-human TLR4 antibody consisting of the amino acid sequence of amino acid numbers 491 to 609 of SEQ ID NO:
• 4, and a polynucleotide comprising a base sequence encoding a light chain variable region of an anti-human TLR4 antibody consisting of the amino acid sequence of amino acid numbers 625 to 732 of SEQ ID NO:
• 4, and an expression vector comprising a polynucleotide comprising a base sequence encoding a light chain variable region of an anti-human TLR2 antibody consisting of the amino acid sequence of amino acid numbers 1 to 113 of SEQ ID NO:
• 6;
• (b) a host cell transformed with an expression vector comprising a polynucleotide comprising a base sequence encoding a heavy chain variable region of an anti-human TLR2 antibody consisting of the amino acid sequence of amino acid numbers 1 to 120 of SEQ ID NO:
• 4, a polynucleotide comprising a base sequence encoding a heavy chain variable region of an anti-human TLR4 antibody consisting of the amino acid sequence of amino acid numbers 491 to 609 of SEQ ID NO:
• 4, a polynucleotide comprising a base sequence encoding a light chain variable region of an anti-human TLR4 antibody consisting of the amino acid sequence of amino acid numbers 625 to 732 of SEQ ID NO:
• 4, and a polynucleotide comprising a base sequence encoding a light chain variable region of an anti-human TLR2 antibody consisting of the amino acid sequence of amino acid numbers 1 to 113 of SEQ ID NO:
• 6;
• and
• (c) a host cell transformed with an expression vector comprising a polynucleotide comprising a base sequence encoding a heavy chain variable region of an anti-human TLR2 antibody consisting of the amino acid sequence of amino acid numbers 1 to 120 of SEQ ID NO:
• 4, a polynucleotide comprising a base sequence encoding a heavy chain variable region of an anti-human TLR4 antibody consisting of the amino acid sequence of amino acid numbers 491 to 609 of SEQ ID NO:
• 4, and a polynucleotide comprising a base sequence encoding a light chain variable region of an anti-human TLR4 antibody consisting of the amino acid sequence of amino acid numbers 625 to 732 of SEQ ID NO:
• 4, and a host cell transformed with an expression vector comprising a polynucleotide comprising a base sequence encoding a light chain variable region of an anti-human TLR2 antibody consisting of the amino acid sequence of amino acid numbers 1 to 113 of SEQ ID NO:
• 6.

A bispecific antibody binding to human TLR2 and human TLR4, comprising:

• 1) a heavy chain variable region of an anti-human TLR2 antibody comprising CDR1 consisting of the amino acid sequence of amino acid numbers 31 to 35 of SEQ ID NO:
• 4, CDR2 consisting of the amino acid sequence of amino acid numbers 50 to 66 of SEQ ID NO:
• 4 and CDR3 consisting of the amino acid sequence of amino acid numbers 99 to 109 of SEQ ID NO:
• 4, and a light chain variable region of an anti-human TLR2 antibody comprising CDR1 consisting of the amino acid sequence of amino acid numbers 24 to 39 of SEQ ID NO:
• 6, CDR2 consisting of the amino acid sequence of amino acid numbers 55 to 61 of SEQ ID NO:
• 6 and CDR3 consisting of the amino acid sequence of amino acid numbers 94 to 102 of SEQ ID NO:
• 6;
• and
• 2) a heavy chain variable region of an anti-human TLR4 antibody comprising CDR1 consisting of the amino acid sequence of amino acid numbers 521 to 525 of SEQ ID NO:
• 4, CDR2 consisting of the amino acid sequence of amino acid numbers 540 to 556 of SEQ ID NO:
• 4 and CDR3 consisting of the amino acid sequence of amino acid numbers 589 to 598 of SEQ ID NO:
• 4, and a light chain variable region of an anti-human TLR4 antibody comprising CDR1 consisting of the amino acid sequence of amino acid numbers 648 to 658 of SEQ ID NO:
• 4, CDR2 consisting of the amino acid sequence of amino acid numbers 674 to 680 of SEQ ID NO:
• 4 and CDR3 consisting of the amino acid sequence of amino acid numbers 713 to 721 of SEQ ID NO:
• 4.