US 2016/0355541 A1 · DISULFIDE-LINKED REVERSIBLE TERMINATORS

Abstract

The present disclosure provides methods of sequencing polynucleotides and compounds, compositions useful for sequencing of polynucleotides. The chemical compounds include nucleotides and their analogs which possess a sugar moiety comprising a cleavable chemical group capping the 3′-OH group and a base that is attached to a detectable label through a cleavable linker comprising a disulfide bond. In addition, both the disulfide bond and the cleavable chemical group are cleavable by a chemical reagent. Furthermore, after the disulfide bond is cleaved by the chemical reagent, there is no free thiol group linked to the base of the nucleotides.

Claims25

Dependent claims

A nucleoside 5′-triphosphate analog having 1) a sugar moiety comprising a cleavable chemical group capping the 3′-OH group of the sugar, 2) a base that is attached to a detectable label through a cleavable linker, wherein:

the cleavable linker comprises a disulfide bond;
both the disulfide bond and the cleavable chemical group are cleavable by a chemical reagent;
and
after the disulfide bond is cleaved by the chemical reagent, there is no free thiol group linked to the base.

1 more element

Dependencies are inferred from the claim text.

Facts

Application

15/150,166Filed 9 May 2016

Priority

8 May 2015

Published

8 Dec 2016

Term ends

Not estimatedThe source records don’t support an estimate.

Classification7 codes

C07H 19/10
Pyrimidine radicals: with the saccharide radical esterified by phosphoric or polyphosphoric acids
C07H 19/14
Heterocyclic radicals containing only nitrogen atoms as ring hetero atom: pyrrolo-pyrimidine radicals
C07H 19/20
Purine radicals: with the saccharide radical esterified by phosphoric or polyphosphoric acids
C12Q 1/6869
Involving nucleic acids: methods for sequencing

Disulfide-linked reversible terminators

Life of this patent

Disulfide-linked reversible terminators

Life of this patent

Abstract

Claims25