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US 2016/0355540 A1Published application
5-position modified pyrimidines and their use
AssigneeSomaLogic, Inc.
InventorsJohn Rohloff, Nebojsa Janjic, Jeffrey D. Carter, Catherine Fowler
Life of this patent
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Abstract
The present disclosure relates to the field of nucleic acid chemistry, specifically to 5-position modified uridines as well as phosphoramidite and triphosphate derivatives thereof. The present disclosure also relates to methods of making and using the same.
Claims3
A C-5 modified aminocarbonylpyrimidine having the following structure:
- wherein
- R′ is selected from the group consisting of —H, —Ac, —Bz, —CH2CH2OCH3, —C(O)CH2OCH3 and —SiMe2tBu;
- R″ is selected from the group consisting of H, DMT and triphosphate (—P(O)(OH)—O—P(O)(OH)—O—P(O)(OH)2) or a salt thereof;
- X is selected from the group consisting of —H, —OH, —OMe, —O-allyl, —F, —OEt, —OPr, —OCH2CH2OCH3 and -azido,
- R is selected from the group consisting of —(CH2)n—RX1;
- RX1 is selected from the group consisting of:
- RX4 is selected from the group consisting of a Cl and a nitrile (CN);
- and
- n=0-10.
6 more elements
A 3′-phosporamidite of a C-5 modified aminocarbonylpyrimidine having the following structure:
- wherein
- R″ is selected from the group consisting of H, DMT and triphosphate (—P(O)(OH)—O—P(O)(OH)—O—P(O)(OH)2) or a salt thereof;
- X is selected from the group consisting of —H, —OH, —OMe, —O-allyl, —F, —OEt, —OPr, —OCH2CH2OCH3 and -azido,
- R is selected from the group consisting of —(CH2)n—RX1;
- RX1 is selected from the group consisting of:
- RX4 selected from the group consisting of a Cl and a nitrile (CN);
- and
- n=0-10.
5 more elements
A 5′-triphosphate of a C-5 modified aminocarbonylpyrimidine having the following structure:
- wherein
- R′ is selected from the group consisting of —H, —Ac, —Bz, —CH2CH2OCH3, —C(O)CH2OCH3 and —SiMe2tBu;
- X is selected from the group consisting of —H, —OH, —OMe, —O-allyl, —F, —OEt, —OPr, —OCH2CH2OCH3 and -azido,
- R is selected from the group consisting of —(CH2)n—RX1;
- RX1 is selected from the group consisting of:
- RX4 selected from the group consisting of a Cl and a nitrile (CN);
- and
- n=0-10.
5 more elements
Dependencies are inferred from the claim text.