Back to results
US 2016/0354739 A1Published application
Device and method for production and analysis of prions
AssigneeSeNostic Health GmbH
InventorsThorsten Luehrs, Felix Deluweit
Life of this patent
Estimating the term…
Loading patent…
Loading patent…
AssigneeSeNostic Health GmbH
InventorsThorsten Luehrs, Felix Deluweit
Estimating the term…
The invention provides a method for producing prion protein having an aggregated conformation by contacting native conformation prion protein with aggregated conformation prion protein in a liquid preparation and subjecting this to at least one cycle or to a number of cycles of application of shear-force for fragmenting aggregates of prion protein, wherein the shear-force applied is precisely controlled. In addition to this process for amplification of aggregated state prion protein from native conformation prion protein, the invention relates to the aggregated state prion protein obtained by the amplification process, which aggregated state prion protein has one conformation, which is e.g. identical within one batch and reproducible between batches, e.g. as detectable by proteinase resistance in a Western blot.
Device for use as a controlled intensity shear-force generator in a process for production of aggregated conformation prion protein from native conformation prion protein by contacting native conformation prion protein with aggregated conformation prion protein in a liquid composition and subjecting the liquid composition to at least one cycle comprising the generation of shear force and a resting phase, the device comprising a shear-force generator arranged to exert a shear-force to each volume element of a liquid composition containing native conformation prion protein and aggregated conformation prion protein, wherein the shear-force generator is controlled to generate a shear-force acting on each volume element of the liquid composition only at one uniform shear force intensity which is limited to an intensity range of maximally 10% of the maximum shear-force.
Aggregated conformation prion protein obtainable by a process for production of aggregated conformation prion protein from native conformation prion protein by contacting native conformation prion protein with aggregated conformation prion protein in a liquid composition and subjecting the liquid composition to at least one cycle comprising the generation of shear force and a resting phase, wherein the shear force is applied to each volume element of the liquid composition and consists of one uniform shear-force intensity having an intensity range of maximally 10% of one shear-force value, wherein the aggregated conformation prion protein is of one amino acid sequence.
Dependencies are inferred from the claim text.