Scaffold for Tissue Growth and Repair
AssigneeNew Jersey Institute of Technology
InventorsTreena Arinzeh, George Collins, Yee-Shuan Lee
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Abstract
Provided is an electroactive structure and method for growing isolated differentiable cells comprising a three dimensional matrix of fibers formed of a biocompatible synthetic piezoelectric polymeric material, wherein the matrix of fibers is seeded with the isolated differentiable cells and forms a supporting scaffold for growing the isolated differentiable cells, and wherein the matrix of fibers stimulates differentiation of the isolated differentiable cells into a mature cell phenotype on the structure.
Claims20
An electroactive structure for growing and differentiating a differentiable cell comprising a three dimensional matrix of electrospun biocompatible synthetic piezoelectric polymer fibers formed by electro spinning the polymer at a high electric potential, wherein the fiber matrix forms a scaffold for supporting cell growth and differentiation;
- and wherein the scaffold conditions are sufficient to induce differentiation of a mesenchymal stem cell into either an osteogenic or chondrogenic phenotype.
An implantable electroactive scaffold produced according to the steps comprising:
- (a) electropsinning a biocompatible synthetic piezoelectric polymeric material at at least 25 kV to form a three-dimensional matrix of piezoelectric fibers, wherein the fibers have an average fiber diameter of from about 500 nm to about 5 μm;
- (b) annealing the electrospun piezoelectric polymeric fibers;
- and
- (c) seeding the annealed three-dimensional matrix of piezoelectric fibers with a mesenchymal stem cell under conditions sufficient to induce differentiation of a mesenchymal stem cell into either an osteogenic or chondrogenic phenotype.
Dependencies are inferred from the claim text.