Methods and compositions for protection against lentiviral infections
AssigneeThe Scripps Research Institute
InventorsMatthew Gardner, Michael Farzan
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Abstract
The present invention provides methods and compositions for optimally co-expressing in a primate subject a tyrosylprotein sulfotransferase (TPST) and a lentiviral gp120-binding molecule to provide potent and long term protection against lentiviral infections.
Claims19
A therapeutic composition comprising (a) a first polynucleotide sequence expressing a tyrosylprotein sulfotransferase (TPST), and (b) a second polynucleotide sequence expressing a binding molecule that, upon tyrosine-sulfation by the TPST, is capable of binding to a gp120 protein of a primate lentivirus or neutralizing infection by the primate lentivirus;
- wherein the molar ratio of the first polynucleotide sequence to the second polynucleotide sequence, or molar ratio of the TPST expressed from the first polynucleotide sequence to the binding molecule expressed from the second polynucleotide sequence, is greater than 1:10.
An expression system, comprising a polynucleotide sequence that expresses a first polypeptide and a second polypeptide at a molar ratio is greater than 1:10, wherein the first polypeptide is a tyrosine-protein sulfotransferase (TPST) or an enzymatic fragment thereof, and the second polypeptide is a binding molecule that, upon tyrosine-sulfation by the TPST, is capable of binding to a coreceptor binding site on a gp120 protein of a primate lentivirus or neutralizing infection by the primate lentivirus
Dependencies are inferred from the claim text.