Gas Evacuation System for Nanofluidic Biosensor

A nanofluidic biosensor system comprising:

• a bottom substrate and a top substrate, an input lateral aperture, a nanoslit having a functionalized area, and at least one of an output lateral aperture and an internal reservoir being arranged between the bottom substrate and the top substrate;
• and
• a gas evacuation subsystem located between the nanoslit and an external environment of the biosensor,
• wherein the nanofluidic biosensor is configured to let a solution having biomolecules enter the input lateral aperture and successively pass through the nanoslit and the at least one of the output lateral aperture and internal reservoir.

The method for detecting biomolecules with a nanofluidic system, the nanofluidic system comprising,

• a bottom substrate and a top substrate, an input lateral aperture, a nanoslit having a functionalized area, and at least one of an output lateral aperture and an internal reservoir being arranged between the bottom substrate and the top substrate;
• a gas evacuation subsystem located between the nanoslit and an external environment of the biosensor;
• and
• an optical system,
• wherein the method comprises the steps of:
• entering a solution having the biomolecules through the input lateral aperture and successively passing the solution through the nanoslit and the at least one of the output lateral aperture and internal reservoir;
• and
• detecting specific biomolecules immobilized on biomarkers inside the nanoslit by quantifying fluorophores attached to the biomolecules of the solution.