Fluidic systems for analyses

A solid-phase assay system comprising:

• a first substantially flat and rigid substrate having one or more detection zones, wherein the one or more detection zones contains a nucleic acid reagent immobilized on the surface of the one or more detection zones, and wherein contacting a sample to the one or more detection zones allows binding between one or more nucleic acids in the sample and one or more nucleic acid reagents;
• a first optical detector aligned with one or more of the detection zones on the first substrate and connected to the electronics of a first reader configured to detect and report a first optical signal related to the binding of one or more nucleic acids to the nucleic acid reagents on the detection zones;
• a second substantially flat and rigid substrate having two or more reaction areas that are not in fluid communication with each other, each reaction area having at least one detection zone, wherein one or more of the detection zones contains a protein reagent immobilized on the surface of the detection zone, and wherein contacting a sample to the detection zones allows binding between one or more proteins in the sample and one or more protein reagents;
• and
• a second optical detector aligned with one or more of the detection zones on the second substrate and connected to the electronics of a second reader configured to detect and report a second optical signal related to the binding of one or more proteins to the protein reagents on the detection zones.