Back to results
US 2016/0260598 A1Published application
Laser enabled imaging mass cytometry
AssigneeFluidigm Canada Inc.
InventorsAlexander V. Loboda, Vladimir I. Baranov
Life of this patent
Estimating the term…
Abstract
The invention relates to methods and devices for analysis of samples using laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS). The invention provides methods and devices in which individual ablation plumes are distinctively captured and transferred to the ICP, followed by analysis by mass cytometry.
Claims28
A laser ablation mass cytometer comprising:
- a laser ablation source for generating ablated plumes from a sample;
- a laser that emits a laser beam;
- an inductively coupled plasma (ICP) torch;
- an injector adapted to couple the laser ablation source with an ICP produced by the ICP torch;
- the injector having an injector inlet positioned within the laser ablation source,
- the injector inlet configured for capturing the ablated plume as the ablated plume is generated, wherein the injector inlet has the form of a sample cone and wherein the diameter of the aperture of the injector inlet is at or between 0.2 mm and 1 mm;
- and
- a gas inlet coupled to the injector inlet and configured to pass a gas from the gas inlet to the injector inlet for transferring the captured ablated plume into the ICP.
5 more elements
A laser ablation cell comprising:
- a) a laser transparent window to allow laser energy to strike a sample to be analyzed;
- b) a stage to hold the sample to be analyzed, wherein the stage is movable in the x-y or x-y-z dimensions;
- c) an injector comprising an injector inlet proximal to the stage, wherein the injector inlet is configured to capture an ablated plume from the sample as the ablated plume is generated, wherein the injector inlet has the form of a sample cone and wherein the diameter of the aperture of the injector inlet is at or between 0.2 mm and 1 mm;
- and
- d) a gas inlet coupled to the injector inlet of the injector inlet and configured to pass a gas from the gas inlet to the injector inlet for transferring the ablated plume into the ICP.
2 more elements
Dependencies are inferred from the claim text.