Methods to detect myocardial injury and uses thereof
AssigneeWashington University
InventorsWalter A. Boyle, Richard Bach
Life of this patent
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Abstract
The present invention provides an accurate and reliable method to detect acute cardiovascular syndromes or disorders and to detect acute cardiovascular syndromes or disorders at earlier time points such that more aggressive interventions can be used in high risk subjects
Claims20
A method to detect acute cardiovascular syndrome or disorder in a subject, the method comprising:
- a) detecting a level of cardiac troponin I (cTnI), fatty acid binding protein (FABP3) and ventricular myosin alkali light chain (MYL3) in a biological sample obtained from the subject;
- b) comparing the level of cTnI, FABP3 and MYL3 detected in (a) to a reference level;
- and
- c) detecting an acute cardiovascular syndrome or disorder when the level of cTnI, FABP3 and MYL3 is significantly increased relative to the reference level.
A method to determine the time that has elapsed since onset of an acute cardiovascular syndrome or disorder in a subject, the method comprising:
- a) detecting a level of cardiac troponin I (cTnI), fatty acid binding protein (FABP3) and ventricular myosin alkali light chain (MYL3) in a biological sample obtained from the subject at about time 0, about time 30 min and about time 60 minutes;
- b) comparing the levels of cTnI, FABP3 and MYL3 detected at about time 0, about time 30 min and about time 60 minutes;
- and
- c) detecting the time that has elapsed since onset of the acute cardiovascular syndrome or disorder, wherein:
- i) a very early period (<1 hour) is detected by low cTnI levels, a positive rate of change and a positive change in the rate of change in cTnI, a positive rate of change and a positive change in the rate of change in FABP3, and a positive then negative rate of change and change in the rate of change in MYL3;
- ii) an early period (<4 hours) is detected by a positive rate of change and a positive change in the rate of change in cTnI, a positive rate of change in FABP3, and a positive then negative change in the rate of change in FABP3;
- and
- iii) a later period (>8 hours) is detected by high cTnI levels, a negative rate of change in cTnI and an absent rate of change and change in rate of change in FABP3 and MYL3.
A method to differentiate between a large and a small acute myocardial injury (AMI) in a subject, the method comprising:
- a) detecting a level of cardiac troponin I (cTnI), fatty acid binding protein (FABP3) and ventricular myosin alkali light chain (MYL3) in a biological sample obtained from the subject at about time 0, about time 30 min and about time 60 minutes;
- b) comparing the levels of cTnI, FABP3 and MYL3 detected at about time 0, about time 30 min and about time 60 minutes;
- and
- c) detecting a large AMI when the cTnI levels at 16-24 hours are greater than about 20 ng/ml and/or when the FABP3 levels are significantly elevated compared to a reference level and/or when the rate of change of cTnI and/or FABP3 are significantly elevated compared to a reference level.
Dependencies are inferred from the claim text.