Screening method for rapid identification of hybridomas
AssigneeTunghai University
InventorsChang-Chi HSIEH, Ming-Shiou JAN, Ai SHIH, Chuan-Kai CHOU, Yen-Wan HSIAO, Ying-Chun LAI
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Abstract
This present invention relates to a screening method for rapidly identifying the hybridomas upon cell size and the expression of an exogenous label such as fluorescence labeling. This method of this present invention can largely shortens the time cost for antibody development by saving the time period with comparison of traditional methods using cell culture with media such as HAT medium.
Claims10
A screening method for rapid identification of hybridomas includes fusing an antigen-immunized splenocyte and a myeloma cell by cell fusion method, analyzing the cell size and the expression of an exogenous label of a cell for selecting a hybridoma;
- wherein the characteristics in that the hybridoma expressing the exogenous label and being larger than the splenocyte and the myeloma cell.
Dependencies are inferred from the claim text.