Method of detecting an analyte in a sample

A composition, comprising:

• (a) a first capture agent that specifically binds a target analyte;
• and
• (b) a reporter complex, comprising:
• a first specific binding member linked to a second capture agent that specifically binds the target analyte;
• and
• a second specific binding member complementary to the first specific binding member, wherein the first and second specific binding members are specifically bound to form the reporter complex.

A composition, comprising:

• (a) a reporter complex, comprising:
• a first specific binding member linked to a first capture agent that specifically binds a target analyte;
• and
• a second specific binding member complementary to the first specific binding member, wherein the first and second specific binding members are specifically bound to form the reporter complex;
• and
• (b) a displacement binding member that is complementary to one of the target analyte, the first capture agent, the first specific binding member, and the second specific binding member.

A system comprising:

• an electrophoresis device comprising an analyte capture zone that comprises;
• a first capture agent that specifically binds either directly or indirectly to a target analyte;
• and
• a reporter complex, comprising:
• a first specific binding member linked to a second capture agent that specifically binds the target analyte;
• and
• a second specific binding member complementary to the first specific binding member, wherein the first and second specific binding members are specifically bound to form the reporter complex.

A kit, comprising:

• a first capture agent that specifically binds a target analyte;
• a reporter complex, comprising:
• a first specific binding member linked to a second capture agent that specifically binds the target analyte;
• and
• a second specific binding member complementary to the first specific binding member, wherein the first and second specific binding members are specifically bound to form the reporter complex;
• and
• a displacement binding member that is complementary to one of the target analyte, the first capture agent, the second capture agent, the first specific binding member, and the second specific binding member.