Back to results
US 2016/0258931 A1Published application
Method for selecting cancer treatment regimen
AssigneeH. Lee Moffitt Cancer Center and Research Institute, Inc.
InventorsAriosto S. Silva, Zayar P. Khin
Life of this patent
Estimating the term…
Abstract
Disclosed is a method for selecting a cancer treatment regimen for a subject.
Claims44
A method comprising
- (a) culturing a plurality of cells from a subject in a chamber;
- (b) contacting the cells in the chamber with an active agent;
- (c) capturing a first optical signal from the cells contacted with the active agent at a first time point;
- (d) capturing a second optical signal from the cells contacted with the active agent at a second time point;
- (e) analyzing the first optical signal and the second optical signal to detect cell membrane motion of the cells;
- and
- (f) analyzing the cell membrane motion to quantify the viability of the cells following contact with the active agent.
4 more elements
A method comprising:
- (a) preparing a dose-response curve by assessing the viability of a plurality of cells from a subject in response to a an active agent at a plurality of time points;
- (b) utilizing the dose-response curve to develop a multi-parameter model, wherein the multi-parameter model describes the rate of accumulation of damage in the cells due to the active agent and the active agent-induced cell death due to the accumulated damage;
- (c) utilizing the multi-parameter model and the dose-response curve to determine the number of populations in the sample;
- and
- (d) utilizing the number of populations and the multi-parameter model to predict a response of the subject to the active agent.
2 more elements
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
A method for selecting a cancer treatment regimen for a subject, comprising:
- (a) administering cancer cells from the subject to a microfluidic chamber that recapitulates the cancer microenvironment and comprises a linear drug gradient across the chamber;
- (b) identifying cell death induced by the drug across the drug gradient at one or more time points;
- (c) collecting parameters from the assay to generate a multi-parameter model that summarizes the response of the subject to the drug treatment;
- and
- (d) selecting a cancer treatment regimen for the subject based on the results of the multi-parameter model.
2 more elements
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
(canceled)
Dependencies are inferred from the claim text.