Compounds and methods for peg metabolite and peg breakdown product assays
AssigneeColonaryconcepts LLC
InventorsKevin DOUGLASS, David HUMPHRIES, Beauregard MASON, Mario PELLERIN
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Abstract
Disclosed herein are compounds and methods for identifying and quantifying a metabolite or breakdown product of PEG. A sample may be assayed for PEG metabolites or breakdown products using liquid chromatography combined with mass spectrometry. Derivatization of the PEG metabolites or breakdown products within the sample with pentaflurobenzoyl chloride in conjunction with negative chemical ionization mode liquid chromatography optimizes the assay.
Claims29
A method for detecting and quantifying a compound, said method comprising
- obtaining a sample, said sample containing one or more of ethylene glycol and diethylene glycol;
- combining said sample, an aqueous hydroxide containing salt, and pentaflurobenzoyl chloride dissolved in an organic solvent thus producing a biphasic liquid system, said biphasic liquid system comprising a pentafluorobenzoate ester derivative;
- separating a liquid phase supernatant from said biphasic liquid system;
- and
- analyzing said supernatant using liquid chromatography and mass spectrometry (LC/MS) to detect said pentafluorobenzoate ester derivative.
The compound ethane-1,2-diylbis(2,3,4,5,6-pentafluorobenzoate).
The compound ethane-1,2-diylbis(2,3,4,5,6-pentafluorobenzoate).
Dependencies are inferred from the claim text.