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US 2016/0258903 A1Published application
Electrophoresis and electroblotting systems and methods
AssigneeBio-Rad Laboratories, Inc.
InventorBoaz Ran
Life of this patent
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Abstract
Integrated electrophoresis and electroblotting systems and methods are provided. In one embodiment, a cassette includes a separation gel and a sheet adjacent to a surface of the separation gel, wherein the sheet is configured to be removed from the cassette without opening the cassette. Systems and methods are also described and illustrated.
Claims18
A cassette comprising:
- a separation gel;
- and
- a sheet adjacent to a surface of the separation gel, wherein the sheet is configured to be removed from the cassette without opening the cassette.
A method comprising:
- removing a sheet from a cassette having a separation gel with previously separated molecules, wherein the sheet is configured to be removed from the cassette without opening the cassette;
- and
- responsive to applying an electric field normal to an electrophoretic plane of the separation gel, transferring the molecules to a membrane.
A system comprising:
- a cassette comprising:
- a separation gel;
- a sheet adjacent to a surface of the separation gel, wherein the sheet is configured to be removed from the cassette without opening the cassette;
- a membrane sandwiched between the sheet and a pad;
- a first and second set of electrodes;
- and
- circuitry operably connected to the first and second set of electrodes and configured to apply a first electric field within an electrophoretic plane of the separation gel and a second electric field normal to the electrophoretic plane of the separation gel.
4 more elements
Dependencies are inferred from the claim text.