Mulitplex assay for the detection of citrus pathogens
AssigneeThe Regents of the University of California
InventorJinbo Wang
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Abstract
The present invention provides methods and compositions for detecting multiple citrus pathogens using a multiplex branched DNA signal amplification reaction.
Claims12
A reaction mixture for detecting the presence of at least one citrus pathogen selected from Citrus tristeza virus (CTV) universal, CTV genotype T30, CTV genotype VT, Citrus psorosis virus, Citrus tatter leaf virus, Citrus leaf blotch virus, Citrus exocortis viroid, Hop stunt viroid, or Citrus leprosis virus in a plant sample, wherein the reaction mixture comprises at least one capture extender probe and at least one label extender probe that target the pathogen, where the at least one capture extender probe and at least one label extender probe each comprise 8, 9, or 10 contiguous nucleotide of a probe sequence as shown in Table 1.
A kit for detecting the presence of at least one citrus pathogen selected from Citrus tristeza virus (CTV) universal, CTV genotype T30, CTV genotype VT, Citrus psorosis virus, Citrus tatter leaf virus, Citrus leaf blotch virus, Citrus exocortis viroid, Hop stunt viroid, or Citrus leprosis virus in a plant sample, wherein the kit comprises at least one capture extender probe and at least one label extender probe that target the pathogen, where the at least one capture extender probe and at least one label extender probe comprises 8, 9, or 10 contiguous nucleotide of a probe sequence as shown in Table 1.
Dependencies are inferred from the claim text.