Back to results
US 2016/0258019 A1Published application
Biomarkers for predicting risk of acute ischemic stroke and methods of use thereof
AssigneeThe Research Foundation for the State University of Newyork
InventorsAlison E. BAIRD, Mateusz ADAMSKI
Life of this patent
Estimating the term…
Abstract
Biomarkers useful for the diagnosis and treatment of acute ischemic stroke are disclosed. Also provided is a quantitative assay method for accurately identifying transcript number in a biological sample.
Claims28
A method for identifying patients having an increased risk for acute ischemic stroke, comprising:
- a) obtaining a biological sample from said patient,
- b) determining the expression levels of at least three genes from Table 4, wherein upregulation of said markers relative to predetermined control levels observed in non-afflicted controls, are indicative of an increased risk for the development of acute ischemic stroke.
A method for identifying agents which useful for the treatment of acute ischemic stroke, comprising:
- a) contacting a cell comprising one or more ischemic stroke associated markers from Table 4 with a test agent;
- and
- b) assessing the effect of said agent on modulation of expression levels of said markers relative to untreated cells, agents which modulate expression of any of the markers in step a) having utility for the treatment of acute ischemic stroke.
A method for quantitative analysis of standard and high-throughput qPCR expression data based on input sample quantity, comprising
- a) obtaining a biological sample for analysis of target gene expression from a test subject and a control subject;
- b) measuring input quantity of said samples and extracting RNA transcripts;
- c) reverse transcribing said extracted RNA transcripts into cDNA and amplifying said cDNA via polymerase chain reaction;
- d) determining amplification efficiency and correlation coefficients for each amplified transcript, wherein relative fold changes between said test and control are calculated using
- T
- C
- C
- C
- =
- ccC
- ccT
- ×
- (
- 1
- +
- E
- )
- (
- nCq
- ,
- C
- -
- nCq
- ,
- T
- )
- wherein, when cells are used as the biological sample, Tc is the number of transcripts per cell in said test subject, Cc is the number of transcripts per cell in a control subject, ccT is the input cell count for said test subject, ccC is the cell input for said control subject, E is the efficiency of target cDNA amplification, and nCq is the cycle number at which amplification crosses the threshold.
24 more elements
Dependencies are inferred from the claim text.