Indicator Molecules For Use In Detecting Enzyme Cleavage Activity
AssigneeMologic Limited
InventorsPaul Davis, Gita Parekh
Life of this patent
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Abstract
The present invention relates to indicator molecules containing multiple cleavage sites for use in detecting enzyme cleavage activity. The invention also relates to various applications of these indicator molecules, for example in enzyme detection devices, particularly although not exclusively, devices for use in the detection of enzyme activity in a test sample. The invention also relates to using the indicator molecules in methods for detecting the presence of enzyme activity in a test sample.
Claims23
An indicator molecule for use in the detection of enzyme cleavage activity in a test sample comprising:
- (i) an enzyme modifiable region comprising multiple separate cleavage sites;
- and
- (ii) a fluorophore,
- wherein cleavage at one or more of the multiple separate cleavage sites causes a measurable change in fluorescence of the fluorophore.
8-18. (canceled)
A method of detecting enzyme cleavage activity in a test sample, the method comprising:
- a. bringing an indicator molecule into contact with the test sample, said indicator molecule comprising
- i. an enzyme modifiable region comprising multiple separate cleavage sites;
- and
- ii. a fluorophore,
- wherein cleavage at one or more of the multiple separate cleavage sites causes a measurable change in fluorescence of the fluorophore.
- b. detecting cleavage of at least one of the multiple separate cleavage sites by measuring a change in fluorescence of the fluorophore.
27-41. (canceled)
45-46. (canceled)
48-53. (canceled)
Dependencies are inferred from the claim text.